Omeprazole and amoxicillin are both commonly used in the treatment of digestive system diseases and have good clinical effects. Amoxicillin is a broad-spectrum antibacterial drug of penicillin class. Before use, it is necessary to confirm whether it is allergic by asking for allergy history or by skin test. It is mainly used for tonsillitis, upper respiratory tract infection and gastrointestinal tract infection caused by sensitive bacteria. Omeprazole can inhibit gastric acid secretion. The drug enters the cells of gastric wall through blood circulation and obstructs ion channels, thus inhibiting gastric acid secretion, protecting gastric mucosa and alleviating discomfort such as acid reflux, nausea, vomiting and heartburn. Clinical use of amoxicillin with omeprazole, bismuth potassium citrate and other antibiotics, such as levofloxacin, metronidazole and clarithromycin, for the treatment of H. pylori infection is commonly known as the gastric quadruple drug regimen. The two drugs can be used together clinically, but it is not advocated to use them at the same time. Generally, after taking one drug, at least half an hour interval is required before taking the other orally, usually omeprazole is taken on an empty stomach before a meal, and amoxicillin is taken half an hour after a meal. Improperly taking two drugs can easily conflict, affecting the efficacy of the medicine and weakening the therapeutic effect of both. Therefore, it is important to seek the guidance of a professional doctor and follow the doctor’s instructions when taking the medication, and not to change the dosage and time of administration without permission, as this may affect the efficacy of the medication or even cause side effects. During the medication period, pay attention to a light and easily digestible diet, avoid stimulating foods, abstain from alcohol, take rest and drink more water.