1, serious tubal diseases, such as tubal blockage and hydrocele due to pelvic inflammatory disease, tubal tuberculosis with normal endometrium, tubal blockage after ectopic pregnancy, tubal hypoplasia, post tubal ligation, bilateral tubal resection such as ectopic pregnancy, etc. 2.Endometriosis, adenomyosis. 3, immune infertility, such as the presence of anti-sperm antibodies, anti-endometrial antibodies, etc. 4. Male factor, i.e. oligospermia, weak spermia, teratospermia. 5. Infertility of unknown origin. 6. Other causes of infertility for which treatment is ineffective. 7. Those with hereditary diseases requiring pre-transplant diagnosis. 8.Other: such as follicle non-rupture syndrome, etc.