Hepatitis C antibody, or anti-HCV, is a marker of viral infection in the body produced after infection with hepatitis C. Anti-HCV contains IgG and IgM, positive for both may be a recent infection, and positive for IgG may be a previous infection, and the test result is susceptible to the stability of the kit. If anti-HCV is positive and the virus HCV-RNA is negative, it proves that there has been a previous infection with anti-HCV, but now it has been improved and the virus has turned negative, which may be acute hepatitis C. If anti-HCV is positive, it is necessary to check whether the virus is positive or not, and if the virus is positive for the current infection, it is chronic hepatitis C. Anti-HCV positive to test HCV-RNA at the same time, the diagnosis should be a combination of test results from several hospitals.