Allergic diseases (also known as allergic diseases) are considered by the World Health Organization (WHO) to be a major health problem worldwide today. Allergic reactive diseases include: allergic rhinitis, bronchial asthma, allergic dermatitis, urticaria, allergic gastroenteritis and allergic uveitis, and other type I allergic reactions. Allergic reactive diseases are a global health problem and are common worldwide, with a global prevalence of 20-40%, and the number of sufferers is still increasing. The most important inducer of allergic diseases in house dust is the dust mite belonging to Dermatophagoids. In the south of the country, 70-80% of patients with allergic diseases are sensitized to dust mites. The most common and important mites in this type of house dust are house dust mite (Der. pteronyssinus) and dust mite (Der. farine). There are also cross allergens with them, such as Meyné’s mite and storage mite. House dust mites are about 400-500 μm long under the microscope and dust mites are about 300-400 μm long, and are small spider-like animals with eight legs. They grow and reproduce by eating up to 50 million skin scales shed per person per day. Each gram of house dust can contain up to 1350 dust mites, and the most common place where dust mites live, the bed, can contain up to 2 million dust mites. The skin prick test (SPT) is performed by placing a small amount of a highly purified allergen liquid drop on the patient’s forearm and gently pricking the skin surface with a prick needle. If the patient is allergic to the allergen, a red bruise resembling a mosquito bite, an itchy reaction, or a change in color will appear at the site of the prick within 15 minutes. We are basically able to determine the presence of an allergic disease. The skin prick test is now recognized as the most convenient, economical, safe and effective allergen diagnosis method in European countries and the United States, with the advantages of high safety and sensitivity, no pain for the patient, just like a mosquito bite, and both the patient and the doctor can immediately know the test results. Contraindications to SPT: ① diseases that significantly impair the systemic state; ② skin lesions at the test site; ③ those with adrenaline contraindications. Precautions for SPT: ① Avoid using it during pregnancy as much as possible; ② In principle, use it when the disease is asymptomatic or basically asymptomatic; ③ 24-48 hours before the skin test, stop using antihistamines and corticosteroids to prevent the appearance of false negatives; ④ Diagnostic results must be combined with medical history.