Toxoplasma gondii can be diagnosed clinically mainly by pathogenic examination and serological examination, among which the pathogenic examination is difficult and the positive rate is not high, so serological examination is an important auxiliary diagnostic tool widely used at present, that is, mainly by blood sampling.1. Pathogenic examination: mainly by smear staining, some secretion of the patient is taken after centrifugation to take the sediment for smear, and then the smear is tested by conventional staining or immunocytochemistry. The smear is then detected by conventional staining method or immunocytochemistry method. IgM antibodies and IgG antibodies. If maternal peripheral blood is drawn before or during early pregnancy and tested positive for IgM antibodies, it indicates a possible recent Toxoplasma gondii infection with risk of transmission to the fetus and requires active treatment; if it is positive for IgG antibodies, it indicates a previous Toxoplasma gondii infection. If Toxoplasma gondii is detected in a pregnant woman before pregnancy, it is recommended to cure Toxoplasma gondii before preparing for pregnancy; if Toxoplasma gondii is detected in a pregnant woman, active treatment is needed, and the pregnancy needs to be terminated if necessary because it may lead to fetal malformation.