1, Folliculopoietic hormone: It is a glycoprotein hormone secreted by basophilic cells in the anterior pituitary gland, and its main function is to promote follicle development and maturation in the ovary. The concentration of blood FSH is 1.5-10mIU/ml in the preovulatory period, 8-20mIU/ml in the ovulatory period and 2-10mIU/ml in the late ovulatory period. 5-40mIU/ml is generally taken as the normal value. low FSH value is seen during estrogen and progesterone treatment, Silhan’s syndrome, etc. high FSH is seen in premature ovarian failure, ovarian insensitivity syndrome, primary amenorrhea, etc. If FSH is higher than 40mIU/ml, it is not effective for ovulatory drugs like clomiphene. 2. Luteinizing hormone: It is also a glycoprotein hormone secreted by basophilic cells in the anterior pituitary gland, which mainly promotes ovulation (under the synergistic effect of FSH), formation of corpus luteum and secretion of progesterone. The concentration of blood LH is 2-15mIU/ml in the preovulatory period, 30-100mIU/ml in the ovulatory period and 4-10mIU/ml in the late ovulatory period. LH/FSH≥3 is one of the bases for the diagnosis of polycystic ovary syndrome. Prolactin: secreted by the lactotropic cells of the anterior pituitary eosinophils, it is a simple protein hormone whose main function is to promote mammary gland hyperplasia, milk production and lactation. During the non-lactation period, the normal value of blood PRL is 0.08-0.92 nmol/L. Higher than 1.0 nmol/L is considered hyperprolactinemia, and excessive prolactin can inhibit the secretion of FSH and LH, suppress ovarian function and ovulation. 4. Estradiol: secreted by the follicles of the ovaries, its main function is to promote the transformation of the endometrium into a proliferative phase and to promote the development of female secondary sexual characteristics. The concentration of blood E2 is 48-521 picomoles/liter in the preovulatory period, 70-1835 picomoles/liter in the ovulatory period and 272-793 picomoles/liter in the late ovulatory period. Low values are seen in low ovarian function, premature ovarian failure and Silhan’s syndrome. Low blood E2 can make clomiphene ineffective in ovulation promotion. 5. Progesterone: secreted by the corpus luteum of the ovary, its main function is to promote the endometrium to change from the proliferative phase to the secretory phase. The blood P concentration is 0-4.8nmol/L before ovulation and 7.6-97.6nmol/L in late ovulation. Low value of blood P in late ovulation is seen in luteal insufficiency and ovulatory dysfunctional uterine bleeding, etc. 6, testosterone: testosterone in women, 50% by the peripheral androstenedione conversion, adrenal cortex secretion of about 25%, only 25% from the ovaries. The main function is to promote the development of the clitoris, labia and mons pubis. It has an antagonistic effect on estrogen and has a certain effect on systemic metabolism. The normal blood T concentration in women is 0.7-3.1 nmol/L. A high blood T value is called hypertestosteronemia and can cause infertility. In case of polycystic ovary syndrome, the blood T value is also increased. Depending on the clinical manifestations, other hormones will be measured if necessary.