If, during your mammogram, you discover an inaccessible breast lesion such as a small breast nodule, or a calcified breast lesion, then your specialist will recommend observation or biopsy, depending on the diagnostic grading. Once the physician deems that your lesion warrants a biopsy, then you will be faced with the problem of choosing which biopsy method to use. There are two methods of biopsy for suspicious small breast nodules (small lumps): 1. Puncture biopsy is performed with the aid of ultrasound by puncturing a metal double-jacketed needle of varying thickness into the middle of a small lump, and then using the concave nest of the needle tip attachment to cut down a strip of fine tissue. This strip of tissue we call a specimen, which is then sent for pathological examination to obtain a pathological diagnosis. The advantage of this method is that the scars on the skin are small and most lesions can be diagnosed. The disadvantage is that the tissue is broken and there is a certain percentage of missed diagnoses. 2.Surgical biopsy is the traditional surgical biopsy, which requires ultrasound-guided puncture with a metal wire to the center of the mass and hooked by its own inverted hook, which we call mass localization. The skin is then incised and the mass is removed along the wire, including a small amount of normal tissue surrounding the mass along with the pathology. The advantage is that the mass and its surrounding tissues are completely removed and no diagnosis is missed. The disadvantage is that there is a 2-3 cm incision in the skin. Two biopsy methods are also available for suspicious clusters of calcified foci: 1. Puncture biopsy A vacuum-assisted puncture biopsy needle is used to puncture into the clusters of calcified foci with the assistance of a molybdenum target, cut off a thin strip of tissue, and then the specimen is sent for pathologic examination to obtain a pathologic diagnosis. The advantage of this method is also that there is minimal scarring on the skin and most lesions can be diagnosed. The disadvantage is that the tissue is broken and there is a certain percentage of missed diagnosis. 2.Surgical biopsy with wire guidance A wire is used to puncture into the middle of the calcified foci under the guidance of molybdenum target, then the skin is cut open and the clusters of calcified foci including some surrounding normal tissues are removed along the wire, and the pathological examination is performed after the molybdenum target has been taken to confirm the removal. The advantage is that the calcified foci and their surrounding tissues can be completely removed without missing the diagnosis. The disadvantage is that there is also a 2-3 cm incision in the skin. There are advantages and disadvantages to both biopsy methods, and patients can choose based on their knowledge of the disease and the risk of the lesion, or they can take the advice of their trusted physician. The physician should choose the appropriate method based on the condition. If the lesion is already palpable, the current worldwide practice is to perform a biopsy first to clarify the diagnosis and then perform surgery.