Hyperandrogenic syndrome is a disease caused by mutations in the number, morphology, structure and union of chromosomes at the time of pregnancy due to harmful chemicals, X-ray irradiation, the influence of environmental factors, advanced gestational age, and consanguineous marriages. Chromosomal examination of amniotic fluid cells is of great significance in the prenatal diagnosis of superoxide syndrome. Amniotic fluid cell culture chromosome examination determination principle, amniotic fluid cells are fetal skin, digestive tract, respiratory tract and through the genitourinary system shed cells, about 95% are dead cells, so it must be cell culture, so that the live cells are fully proliferated, in order to harvest the cells for karyotype analysis. The operation method is as follows: 1, take 15-30ml of amniotic fluid from 16-20 weeks of gestation, centrifuge at 1000r/ml for 10min. 2, discard the excess supernatant, leave 1ml of amniotic fluid and precipitated cells, and gently break them up into a cell suspension. 3, 3 ml of nutrient base and 1 ml of calf serum, placed in a 37 ℃ incubator culture. 4, culture 7 ~ 10 days after a large number of fibroblast-like or epithelioid cell colonies can be seen. At this time, fresh culture medium can be changed. 5.To be expanded into cell colonies, and there are many translucent round split cells, you can add colchicine, so that the final concentration of 0.1 ~ 0.3μg / ml, placed in 37 ℃ incubator and then cultured for 5 ~ 6h (the above processes are carried out under sterile conditions). Harvesting criteria: ① fibroblasts as the main type of cell growth in patches; ② observed with a 10x eyepiece and 20x objective, the growth of cell clones covering 1 or more than 1 complete field of view; ③ see more than 10 translucent round cells and more than 10 double-rounded bright split cells. 6.If the cell growth is not vigorous, the growth cycle is not aligned or the cell aging, not up to the harvest standard, you can continue to passaging culture in the original bottle. Methods: Pour off the culture medium first under aseptic conditions, add several drops of 2.5g/L sterile trypsin solution, shake and pour off. Then add 5-10 drops of trypsin and shake gently for about 5 min at 37℃ to dislodge the adherent cells. Add 4ml of fresh medium containing calf serum, and incubate at 37℃ for 4~5 h. Then carefully replace the culture medium and continue to incubate, and generally harvest it in 3~5 days after substitution. 7, transfer the culture medium to a graduated centrifuge tube, add 1ml of ED-TA-trypsin solution in the culture flask, and place it in a 37℃ warm box for 5min, then rinse the adherent cells with an elbow pipette (2.5g/L trypsin solution can also be used for digestion). The cells detached from the wall of the bottle were poured into a centrifuge tube, mixed with the original culture medium, and the bottle was rinsed with a small amount of warm saline, and the washed cells were also poured into the centrifuge tube, and centrifuged at 1000r/min for 10min. 8, the supernatant was aspirated and discarded, and 3~5ml of pre-warmed 0.075mol/L KCl hypotonic solution was added at 37℃, and placed at 37℃ for 10~15min. 9, pre-fixation, Fixation and preparation were the same as the preparation method of peripheral blood cell chromosome specimens. Under normal circumstances, the total number of chromosomes is 46; among them, there are 22 pairs of autosomes (serial number 1 to 22); sex chromosomes are XY for males and XX for females; male karyotype is 46, XY; female karyotype is 46, XX. Chromosome examination is applicable to various chromosome number abnormalities: such as Down’s syndrome (trisomy 21), trisomy 18, trisomy 13, trisomy 8, trisomy 22, trisomy 3, congenital syndrome, trisomy 3, congenital syndrome, and trisomy 3, trisomy 3, trisomy 3, trisomy 3, trisomy 3, and trisomy 3. Trisomy 8, Trisomy 22, Congenital testicular hypoplasia syndrome, XXY syndrome, Turner’s syndrome, Trisomy X and Poly X syndrome.