In vitro fertilization process

  Patients often ask about the timeline for IVF and want to know how long it takes in total to do IVF so that they can adjust to the work sooner.  However, the first thing we should know is that the time required to do IVF is closely related to the patient’s specific situation, medication regimen and the doctor’s skills, and the length of time varies, with no uniformly defined standard, generally about 1-3 months.  I. Pre-examination stage Both parties have to do a full set of examination. The usual time is 1-2 months. The male partner needs to have his semen tested and the female partner needs to complete some basic examinations, such as gynecological examination, diagnostic scraping, laparoscopy, anti-sperm antibodies, liver function and hepatitis B and half, routine blood analysis and clotting time and basic endocrine hormone determination, etc. The specific examination items also vary from person to person.  The ovulation promotion and follicle monitoring phase will last for about 1 month. Generally, the medication is started at the mid-luteal phase, i.e. day 21 of menstruation, so that the gonadotropins in the body are at a low level and the menstrual flow occurs in about 8 days, and on day 3-7 of menstruation, intramuscular injection of follicle development drugs is started.  3. Egg retrieval, sperm retrieval, embryo in vitro culture and transfer After about 10 days of follicle development drugs application, the follicles will be mature, at this time, the eggs can be retrieved by ultrasound-guided transvaginal vault puncture. The sperm and eggs are placed in a special medium and fertilized in vitro. After 3 days of culture, the fertilized eggs develop into embryos and are placed into the uterine cavity and rest for a few moments after the transfer.  IV. After embryo transfer After the embryos are developed 3-5 days after egg retrieval, the laboratory embryologist will usually freeze the embryos and the doctor will first condition the endometrium for the patient for 1-2 menstrual cycles, and then transfer them when the endometrium thickness is suitable.  At the time of transfer, the doctor will apply a very thin embryo transfer tube to transfer the best embryos into the mother’s uterus through the cervix. Depending on the age, embryo quality and previous IVF outcomes, the number of embryos to be transferred is decided, usually 1-2 embryos are transferred.  V. Luteal support Due to the application of agonists/antagonists and ovulation-promoting drugs, as well as the loss of follicular granulosa cells due to egg retrieval, women usually have luteal insufficiency during the egg retrieval cycle and require luteal supplementation/support with progesterone and/or chorionic gonadotropin. If there is no pregnancy, discontinue progesterone and wait for the onset of menstruation. If pregnancy occurs, continue progesterone application as appropriate.  VI. Determination of pregnancy Serum HCG is measured about 14 days after embryo transfer to determine if pregnancy is present. Transvaginal ultrasound examination is performed 28 days after embryo transfer to determine whether intrauterine pregnancy is present and whether there is fetal heartbeat.