Changes in the distribution of body fluids during exercise through neurohumoral regulation can lead to significant changes in blood biochemical indicators. During strenuous exercise, potassium, sodium, calcium, alkaline phosphatase, creatine kinase, albumin, sugar, inorganic phosphorus, uric acid, urea bilirubin, and aspartate aminotransferase all increase more than 1-fold, and white blood cell count, pyruvate, and lactate can increase rapidly, while serum non-esterified fatty acids decrease rapidly. Fluctuations in the results of tests associated with them can also result from the effects of local exercise on local organs. For example, if a man rides a bicycle for 30 minutes before the prostate-specific antigen test, it will more than double. Therefore, in order to obtain objective and accurate test results, it is important to take rest and avoid strenuous exercise prior to blood sampling.