Gallbladder carcinoma in situ generally does not need chemotherapy. Carcinoma in situ of gallbladder refers to tumors that are only confined to the mucosal epithelium and have not broken through the basement membrane, and the cancer cells have not infiltrated and metastasized to the mesenchyme or other parts of the gallbladder; most of the gallbladder carcinoma in situ have better prognosis, and the treatment is based on surgical resection, and generally do not need radiotherapy or chemotherapy after the surgery. If there are opportunities and conditions for chemotherapy for malignant tumors, chemotherapy should be chosen as much as possible; however, most of the gallbladder cancer cells do not have high sensitivity to chemotherapy, and if chemotherapy is needed, it is often necessary to use 2~3 kinds of chemotherapeutic drugs, and the adverse reactions will be aggravated accordingly. At present, there is a lack of unified standard chemotherapy program for gallbladder cancer, but if chemotherapy is really needed, it can be based on the chemotherapy program of pancreatic cancer, gastrointestinal cancer, etc.; choose carboplatin, oxaliplatin, cisplatin, fluorouracil, epirubicin, etc.; generally, intravenous drug can be given, or interventional hepatic artery cannulation can be done for local arterial perfusion. The effect of chemotherapy for gallbladder cancer currently lacks more unified understanding and authoritative research results. Chemotherapy can generally shrink the tumor and can be an important choice in the treatment of advanced gallbladder cancer.