Lymph node biopsy is generally performed in the following ways: Surgical excision: the most direct, with the highest positive rate, and this is preferred; the disadvantage is that sometimes the location of the lymph nodes is deep, and surgery has certain difficulties or risks, such as bleeding or infection. Lymph node puncture: Most of the punctures are performed under ultrasound guidance, usually with a coarse needle, and fine needles are rarely used anymore. The disadvantage is that the positive rate is relatively low, the tissue obtained is relatively small, and sometimes the specimen is insufficient for diagnosis; the advantage is that there are few complications and little risk of bleeding. It is usually only taken in cases where the procedure is difficult or risky. Where are lymph node biopsies taken? Patients will often have multiple enlarged lymph nodes, which area should be taken for biopsy? 1. If PETCT has been done, take the area with the highest SUV value. 2.If ultrasound of lymph nodes has been done, choose the area with the largest volume and disrupted structure for biopsy. Of course, the premise is to ensure patient safety.