Assisted reproductive techniques (ART) refers to a series of techniques to treat infertility through manipulation of eggs, sperm, fertilized eggs and embryos. It is divided into two categories: artificial insemination (artificial insemination), in vitro fertilization and embryo transfer (IVF-ET) and derivative techniques. 1, artificial insemination, divided into two categories: artificial insemination with husband’s semen (AIH) and artificial insemination by donor (AID), the former using the husband’s sperm, the latter using donor sperm. 2. In vitro fertilization-embryo transfer and its derivative techniques are assisted reproductive techniques that enable sperm and eggs to meet and fertilize outside the body and then transfer into the mother to achieve pregnancy. The main techniques include conventional IVF-ET, intracytoplasmic sperm injection (ICSI), pre-implantation genetic diagnosis (PGD), embryo Cryopreservation, egg and embryo donation, surrogacy, etc. The history of assisted human reproduction dates back more than 200 years to 1790, when John Hunter applied artificial insemination to solve the fertility problem of an infertile couple with hypospadias. In 1992, Palermo used ICSI to achieve the second generation of IVF, and PGD to achieve the third generation of IVF. The first generation of IVF is the conventional IVF technique, in which the eggs are removed vaginally under ultrasound guidance and fertilized with treated sperm in a Petri dish when the eggs are mature, and then transplanted into the uterine cavity of the woman after they have reached a certain stage of development. The so-called second-generation IVF technique, intracytoplasmic single sperm injection (ICSI), is simply a microinjection technique to fertilize an egg by injecting a sperm directly into the cytoplasm of the oocyte at the time of fertilization. All other procedures are the same as conventional IVF techniques. It is suitable for patients with extreme oligospermia and weak sperm. The so-called third generation IVF technique, known as Pre-implantation Genetic Diagnosis (PGD) technique, only screens the embryos before implantation into the mother to ensure that embryos free of genetic disorders are implanted into the uterus. Embryos are screened to prevent genetic disorders. All other processes are the same as conventional IVF techniques. From the above, it can be seen that the classification of the first, second and third generation IVF techniques is wrong. It is reasonable that the second generation techniques should be able to completely replace the first generation techniques, the fact is that they are just an additional operation to the conventional IVF techniques, just like the relationship between WINDOWS and other application software, the conventional IVF techniques are the technical operation platform. In fact, there are dozens of assisted reproductive technologies (ART), which are variations on conventional IVF technology and cannot be divided by generation. Internationally there is no such thing as the first, second or third generation of IVF technology, only that the country likes it, probably for news hype. There is also a phenomenon that in the international arena, the third generation of IVF technology was successful in 90 years, while the second generation of IVF technology was successful in 92 years.