What is Ebola hemorrhagic fever (III) – Virus structure and biological shape

  Virus structure and biological shape 1, structure morphology Ebola virus (EBV) belongs to the filovirus family, a long filamentous body, single-stranded negative-stranded RNA virus with 18,959 bases and a molecular weight of 4.17 × 10? There is an envelope outside, the virus particle diameter is about 80nm, size 100nm × (300 ~ 1500) nm, the virus with strong infection ability is generally about (665 ~ 805) nm long, branch-shaped, U-shaped, 6-shaped or ring-shaped, branch-shaped is more common. There is a capsule membrane, and the surface has (8-10) nm long fibrils. The pure viral particle consists of a helical ribosomal riboshell complex containing a negative-stranded linear RNA molecule and four virulence particle structural proteins.  2, biological traits “Ebola” virus shape like the ancient Chinese “Ruyi”, the use of electron microscopy study of members of the genus Ebola virus, it shows the general linear structure of fibrovirus. Virus particles may also appear “U”, “6”, twisted, ringed or branched, although laboratory purification techniques may also be a factor in the creation of these shapes, for example, the high speed of centrifuges may distort the virus particles. Viral particles are typically about 80 nm in diameter but can be up to 1400 nm in length, with the average length of a typical Ebola virus particle approaching 1000 nm. The nucleocapsid protein at the center of the viral particle structure consists of a spirally wound genomic RNA with a nucleocapsid protein and the protein viral proteins VP35, VP30, and L. The virus contains a glycoprotein that penetrates 10 nm deep into the viral particle from the surface and another 10 nm protrudes outward on the surface of the envelope, which is derived from the host cell membrane, and the area between the envelope and the nucleocapsid protein, called the matrix space that consists of the viral proteins VP40 and VP24.  EBOV is stable at room temperature, moderate resistance to heat, 56 ℃ can not be completely inactivated, 60 ℃ 30 min to destroy its infectivity; UV irradiation 2 min can make it completely inactivated. EBOV can survive for several weeks in blood samples or corpses; its infectivity remains unchanged for 5 weeks at 4°C, and its titer drops to half in 8 weeks. Long-term storage at 70°C is possible.  Although the natural host of EBOV has not been conclusively identified, there is multiple evidence of EBOV infection in wild nonhuman primates such as monkeys and orangutans, as well as other animals. Evidence 1: Epidemics in 1976, 1996, and 2002 stemmed from human contact with orangutans that died in the wild; Evidence 2: EBOV was detected several times in monkeys exported from the Philippines, but no morbidity was found; Evidence 3: Investigations by the Ministry of Health and Wellness in Congo-Brazzaville in August 2003 showed that EBOV could be detected in chimpanzees, wild boars in the wild.