Methods for detecting mycoplasma include culture, serological methods (including enzyme-linked immunosorbent assay and micro-immunofluorescence test, but rarely used clinically) and biological methods (including DNA probes and PCR), the former often performed simultaneously with drug sensitivity testing. The significance of mycoplasma culture and in vitro drug sensitivity test in clinical diagnosis and treatment is as follows: 1, the clinical suspicion of mycoplasma infection patients, should first culture identification of the pathogen and drug sensitivity test, in order to make a correct diagnosis, select sensitive drugs for reasonable and effective treatment, not only help the rational and safe application of antibiotics, but also help to improve the cure rate, and more help to control the production and spread of drug-resistant strains of bacteria The actual antibiotics can also prevent the economic waste and harm caused by the blind and random application of antibiotics. The actual results of the in vitro drug sensitivity test are different from the in vivo drug activity. The drug sensitivity test before treatment can guide the use of high-sensitivity drugs, and to achieve reasonable, adequate and regular use of drugs. 3, the culture of mycoplasma, the number of ≥ 104, and clinical symptoms, the diagnosis can be confirmed, should be actively treated. If there are no clinical symptoms, the number of mycoplasma ≤ 104, generally can not be treated, but the pro-life should be given treatment as appropriate. 4, in vitro drug sensitivity test contains tetracycline, macrolide and fluoroquinolone three types of antibiotics that do not act on cell wall biosynthesis, if the drug sensitivity results show that the infected strain is not sensitive to the three types of antibiotics on the report card, the use of this drug sensitivity test other than antibiotics or in accordance with the “90”, “60 If the results show that the infected strain is not sensitive to the three types of antibiotics on the report list, the antibiotics other than this drug sensitivity test can be used or in accordance with the “90”, “60” principle, combined with the patient’s drug history and clinical performance, that is, the choice of insensitive antibiotics, there may be 60% of patients to obtain results. 5, medication treatment should be adequate, the course of treatment is generally 10-14 days; persistent and recurrent cases require a 30-day course of treatment. 6, 1 week after completing the treatment course and stopping the drug, recheck mycoplasma, if there is no growth after 72 hours of culture, it is negative, indicating that the disease has been cured. If there is still growth and the number of ≥ 104, indicating that the treatment has failed; after excluding reinfection, should be regarded as a persistent case, then according to its drug sensitivity results, to be retreated, and the course of treatment should be 30 days. In order to improve the culture positivity and accuracy, the following three points should be noted: (1) Antibiotic drugs should be stopped for 5-7 days before taking the specimen. (2) Male patients should preferably take specimens more than 2 hours after urination. (3) When taking the specimen, the cotton swab should be stretched about 2 cm into the urethra or cervical orifice, and the swab should be repeatedly rotated to obtain mucosal epithelial cells, not secretions or leucorrhea, much less urine. The sensitivity and specificity of the PCR method are high, but since the PCR test results may be positive when the dead mycoplasma has not been cleared by the body after the culture has turned negative after treatment, this method is not used clinically to confirm the diagnosis and evaluate the efficacy of the treatment.