Nucleic acid testing, if performed by nasal and pharyngeal swab methods, usually does not recommend that the person to be tested eat, especially within 2 hours before the test, and should avoid eating to avoid vomiting. Smoking, drinking, alcohol, chewing gum, etc. should not be used 30 minutes prior to sampling to avoid frequent swallowing that could affect virus enrichment in the throat. If an anal swab test is performed food is usually allowed and there are no special requirements. However, most nasopharyngeal swabs are still taken for nucleic acid testing for the entire population and for the general population. Although it is not possible to eat before the nucleic acid test, patients can drink a moderate amount of water and it will not affect the test results, but it is not recommended to drink drinks, juices and other beverages, especially alcoholic beverages, which can have an impact on the results. In addition, attention should be paid to the correct wearing of the mask before performing nucleic acid testing. The mask should be removed only when sampling oropharyngeal swabs and worn immediately after sampling, and a spare mask can be prepared for easy replacement at any time after contamination. Carry tissues with you, discomfort may occur during sampling, such as tears and sneezing. In addition, due to the tight protection of medical personnel, may not be able to hear the speech, carry your ID card and open the health code in advance to save time and improve sampling efficiency. Queuing time is relatively long, those to be examined should please listen to the command, consciously keep more than 1 meter interval and do not talk.