After nearly 40 years of development, IVF technology, despite having made great strides, still suffers from lingering success rates: 1. Age: It is well known that female age is an important killer of fertility. For men likewise, around 30 years old male fertility is best, with age, male sperm quality is on the decline. 2, obesity: male weight affects IVF outcomes. Studies have shown that male body mass index (BMI) R25kg/m2, the clinical pregnancy rate of assisted reproduction significantly decreased. 3, deformed sperm: deformed sperm may affect the fertilization rate (including natural conception and assisted reproduction), but deformed sperm has no effect on abnormal fertilization (such as multiple sperm fertilization), the number of embryos obtained, embryo quality, and the success rate of implantation, and has little correlation with embryonic arrest, abnormal fetuses, and preterm birth. 4. Sperm DNA integrity (sperm fragmentation rate, DFI): sperm DNA damage is closely related to IVF outcome, which can cause fertilization failure, embryo abnormalities, and is closely related to miscarriage, preterm birth, and birth defects. Therefore, it is important to pay attention to sperm NDA integrity testing, which is crucial for determining IVF outcomes. It is recommended to be carried out routinely in hospitals that have the conditions. (The main factors leading to sperm DNA damage include, smoking, drinking, staying up late, advanced age, etc.) 5. Sperm RNA testing: Sperm RNA is important for assessing sperm vitality, fertilization ability and developmental potential of embryos after fertilization. Therefore, RNA testing is of some significance to accurately assess male fertility and determine the outcome of IVF. This test is still in the laboratory stage and should be actively carried out in the clinic.