The sex hormones are usually checked by fasting blood on the third day of menstruation, and each increase or decrease represents a different clinical significance. /If the ratio of LH divided by FSH is above 2.5, it indicates polycystic ovary syndrome; for estradiol, if the development of secondary sex characteristics, i.e. breast development, occurs before the age of 8, and the blood estradiol is elevated, it indicates precocious puberty. In addition, elevated estradiol is commonly seen in granulosa cell tumors, ovarian plasmacytoma, systemic lupus erythematosus, obesity, pregnant women with diabetes mellitus, and normal pregnancies; for progesterone, it usually increases after ovulation. Elevated prolactin can be due to the effects of medications, pregnancy or hyperprolactinemia, or pituitary tumors; elevated androgens are usually seen in polycystic ovary syndrome. In conclusion, the sex hormone six tests generally need to be analyzed in conjunction with medical history or relevant tests. Clinically, high FSH, LH, prolactin and androgen are more common, while low progesterone is more common.