Introduction to other IVF prolotherapy protocols Antagonist protocols IVF technology has developed rapidly in recent years, and prolotherapy protocols have become increasingly flexible and varied, and how to treat safely and conveniently for patients without compromising pregnancy rates has become the current direction of IVF treatment development. In addition to the previously introduced IVF treatment and ovulation protocols, this issue introduces several more flexible protocols. Antagonist protocol, which is currently a more flexible protocol for patients with polycystic ovary syndrome and patients with low ovarian function and poor response to previous ovulation promotion. Serum hormones and vaginal ultrasound are measured on the third day of menstruation to assess the basal status of the ovaries, and ovulation promotion is started when conditions are suitable. Antagonist regimen can effectively inhibit the early onset of LH peak and prevent early follicular discharge; reduce the occurrence of ovarian hyperstimulation syndrome and improve the safety of IVF treatment in patients with polycystic ovary syndrome, meanwhile, the cycle time is short, the cost is low and the application is convenient and flexible, however, antagonist regimen requires close monitoring of hormonal changes and follicular growth during ovulation promotion, timely addition of antagonist, and due to the luteal function effect of antagonist However, the antagonist regimen requires close monitoring of hormonal changes and follicular growth during ovulation, timely addition of antagonists, and increased luteal support after transplantation due to the luteal function effects of antagonists.