The diagnosis of azoospermia is usually determined after 2-3 semen examinations and no sperm is found after specimen centrifugation. The absence of sperm in semen is only a superficial phenomenon, and detailed examination is required to further clarify the cause and diagnosis of the condition. The reason for this is three: 1, the manufacture of sperm “factory” shutdown Men’s testicles are sperm-producing factories, the testicles in the fine structure is very complex, are designed for men to pass on to the next generation. The testicles of men are very fragile, very susceptible to various all and local adverse factors, so that the function of sperm production is seriously injured, clinically visible mumps-induced testicular inflammation, bilateral cryptorchidism without timely surgical treatment may lead to sperm dysfunction, patients often need to use artificial insemination treatment to obtain the next generation. 2.The sperm produced cannot be transported out For male patients with normal testicular function, normal endocrine level and obstruction of the reproductive tract who have difficulty in surgical reconstruction, cannot recover or refuse to undergo surgical reconstruction, they can consider removing sperm from the epididymis or testicles and obtain the opportunity to have children through IVF technology. Individuals may not leave all the semen in the semen cup due to nervousness during the examination or the semen is not fully discharged during the sperm retrieval, resulting in abnormalities in the examination. Some primary hospitals do not examine semen specimens carefully, resulting in the diagnosis of “azoospermia”. In order to clarify the cause of azoospermia, the diagnosis must be made through relevant examinations, including the following optional tests in addition to the taking of relevant medical history and detailed physical examination: semen pH, seminal plasma biochemistry (fructose, alpha-neutral glucomannan), sex hormones, transrectal ultrasound, chromosome examination, mycoplasma, chlamydia and bacterial culture.