The main process of conventional IVF includes 1. descending regulation: the purpose is to suppress the LH elevation and LH peak in the pro-superovulation cycle, inhibit ovulation, make the eggs develop better and more synchronized, no early spontaneous ovulation occurs, so that egg retrieval can proceed smoothly; 2. superovulation promotion: the use of drugs to promote the development of multiple follicles in both ovaries to mature and produce high quality eggs. 3. monitoring follicular development: using ultrasound to understand the underlying conditions of the ovaries and uterus and to understand the number and size of growing follicles in order to adjust the dose of medication. In addition, if necessary, blood will be drawn to measure the estrogen level to understand the maturation of the follicles. 4. Egg retrieval: 36 hours after hCG injection, the eggs will be removed under vaginal ultrasound guidance. 5. In vitro fertilization and culture: After egg retrieval, the husband masturbates to retrieve sperm, and in patients with obstructive azoospermia, sperm is retrieved by epididymal or testicular puncture. First generation IVF sperm is processed and cultured with the egg for fertilization; second generation IVF injects sperm into the egg by microinjection method to help fertilization; fertilization is observed after 12~18 hours. 6. Embryo transfer: 2~3 days after egg retrieval, the 2 best developed embryos are selected and sent back to the uterus; blastocyst transfer is performed 5 days after egg retrieval. The remaining better embryos can be frozen and preserved. 7. Luteal support: to allow the embryos to continue to grow and develop in the body for the purpose of pregnancy.