Combining the recommendations of the National Heart Association and the Medical Advisory Board of the Neurological Society on thrombolysis in acute ischemic stroke and the domestic experience, the following recommendations are made: 1. Intravenous thrombolysis with streptokinase is not recommended; genetically recombinant t-PA must be applied within 3 hours of onset; the principle of applying domestic urokinase is no more than 6 hours of onset, total arterial thrombolysis of 250,000 to 750,000 U, contrast time less than 1.5 hours, intravenous thrombolysis The total amount of 500,000-2.5 million U, intravenous drip is less than 2 hours, preferably within 1 hour; thrombolysis is not considered for those who are not sure of the onset time. 2.Recommendation is allowed only after confirmation of diagnosis by a neurologist and evaluation of CT and MRI images by a neuroimaging physician; anyone whose images have shown infarct foci corresponding to physical signs cannot be enrolled. 3. Those who meet the following conditions cannot be enrolled: those with rapidly improving symptoms and signs before thrombolysis; those with seizures that cannot be effectively controlled; those with a single neurological deficit that cannot be scored, such as ataxia, sensory impairment, etc.; those with a history of cerebral hemorrhage; those with a history of cerebral infarction and craniocerebral trauma within 6 months, leaving significant functional deficits; those with severe cardiac, hepatic and renal insufficiency; those with myocardial infarction; those with surgery and childbirth; and those with active peptic ulcer within 6 months. Active peptic ulcer within six months; gastrointestinal or urinary bleeding; known bleeding tendency; oral anticoagulants with prothrombin time >15 s; intravenous heparin within 48 hours prior to the disease with partially prolonged prothrombin time; platelet count <10×109/L, blood glucose <2.8 mmol/L or >22.2 mmol/L, pre-treatment systolic blood pressure >180 mmHg or diastolic blood pressure >100 mmHg. 4. Thrombolysis should be performed with complete emergency adjuvant treatment measures and resuscitation equipment for intracranial hemorrhage. 5. Patients should be informed of the risk of serious bleeding and the possible efficacy before thrombolysis and sign a voluntary letter.