The diagnosis of ovarian cysts and their benign and malignant nature can be confirmed by the following tests: 1. ultrasound examination, ovarian cysts can usually be diagnosed by abdominal ultrasound or vaginal ultrasound, with an accuracy rate of 90%. ultrasound examination should be performed within 1 week of menstrual cleansing, avoiding ovulation as much as possible. This is a better time to determine if the cyst is physiological or pathological. Ultrasound can show the morphology, size, number of ovarian cysts, the nature of the cysts inside, separation and blood flow signals; 2. CT and MRI: CT and MRI can observe the morphology of ovarian cysts more clearly and whether there are lymph node metastases. Most benign cysts have clear and smooth borders and usually no lymph node enlargement, while most malignant tumors have irregular contours and poorly defined boundaries with surrounding tissues, often accompanied by ascites and also lymph node enlargement; 3. tumor marker examination: if the cyst is a malignant ovarian tumor, it, like other tumors, can produce and release antigens, hormones and enzymes, which are in the patient’s serum. These substances can be measured by immunological and biochemical methods, and we call these substances tumor markers. AFP is the best tumor marker for endodermal sinus tumor and is also elevated in patients with immature teratoma. The second is hormonal markers. The next hormonal marker is chorionic gonadotropin, or HCG, which is a highly specific marker for gestational trophoblastic disease. Serum concentrations are also elevated in patients with ovarian choriocarcinoma. In patients with granulosa cell tumor and follicular meningioma, estrogen levels tend to be elevated. 30% of patients with testosteroblastoma have significantly higher urinary 17-ketosteroid excretion. Secondly, the enzyme marker lactate dehydrogenase LDH, the excretion is also increased in patients with ovarian malignancy. 4. Laparoscopy: Laparoscopy can directly see the general condition of the mass, the whole pelvic and abdominal cavity can be observed, there is an intuitive judgment of the morphology and structure of the cyst, the relationship between the cyst and the surrounding tissues, and a multi-point biopsy can be performed in the suspected areas.