Objective: To analyze the level and distribution characteristics of serum 25(OH)VitD in patients undergoing orthopedic spine surgery. Methods: A total of 391 patients, 164 males and 227 females, aged 17-88 years (58.0±13.9), admitted to our orthopedic spine ward for surgical treatment from September 2012 to July 2013 were retrospectively analyzed, and serum 25(OH)VitD was examined preoperatively. 25(OH)VitD < 20ng/ml was considered deficient, 20ng/ml to 32ng/ml was considered deficient, and ≥32ng/ml was considered normal. ml was considered deficient, and ≥32ng/ml was considered normal. The relationship between different factors such as gender, age, type of disease, and season and serum 25(OH)VitD levels was analyzed and discussed. Results: Among 391 patients, 304 (77.75%) were deficient in 25(OH)VitD, 78 (19.95%) were deficient, and 9 (2.30%) were normal, with a mean value of 14.98±7.13 ng/ml. 112 (68.29%) and 44 (26.83%) were deficient and deficient in males, respectively; 192 (84.58%) and 34 (26.83%) were deficient and deficient in females, respectively. 84.58%), 34 cases34 (14.98%). 85 cases (79.44%), 17 cases (15.89%) were deficient and insufficient in patients aged 18-50 years; 141 cases (75%), 45 cases (23.94%) were deficient and insufficient in patients aged 50-70 years; 78 cases (81.25%), 16 cases (16.67%) were deficient and insufficient in patients aged ≥70 years. 16.67%). The proportion of deficiency/insufficiency of each disease type was 59 cases (76.62%)/18 cases (23.38%) for cervical spondylosis, 91 cases (79.82%)/19 cases (16.67%) for lumbar disc herniation, 28 cases (84.85%)/5 cases (15.15%) for lumbar spinal stenosis, 25 cases (65.79%)/13 cases (34.21%) for lumbar spondylolisthesis, and 25 cases (65.79%)/13 cases (34.21%) for spinal Fractures (including osteoporotic compression fractures) 89 (80.18%)/19 (17.12%), spinal revision surgery 9 (60%)/4 (26.67%), tumors 3 (100%)/0 (0.00%). lack of patients in each month from January to December were 29 (90.625%), 15 (83.33%), 23 (79.31% ), 39 (79.59%), 35 (81.40%), 34 (77.27%), 28 (70.00%), 2 (100%), 7 (35.00%), 25 (69.44%), 36 (90.00%), 31 (81.58%); the number of 25(OH) VitD deficiency cases in each month was 3 (9.375%), 3 ( 16.67%), 5 (17.24%), 19 (38.78%), 8 (18.60%), 10 (22.73%), 11 (27.5%), 0 (0%), 9 (45.00%), 9 (25.00%), 4 (10.00%), 6 (15.79%). CONCLUSIONS: The preoperative presence of 25(OH) VitD deficiency and insufficiency in patients undergoing spinal surgery is very serious, and the causes of low VitD levels may be related to the patients' restricted activity and lifestyle, and VitD deficiency may affect the prognosis of patients undergoing surgery.