In vitro fertilization-embryo transfer (IVF) is an artificially assisted reproductive technique to help certain infertile couples achieve pregnancy. The technique includes conventional in vitro fertilization-embryo transfer (IVF-ET), intracytoplasmic microinjection of a single spermatozoon (ICSI) and its derivatives. Conventional in vitro fertilization-embryo transfer (IVF-ET) is to take the egg and sperm out of the body and fertilize them in vitro, and then the fertilized egg will be cultured into an early embryo in vitro and transplanted into the uterus of the female partner for growth and development; intracytoplasmic microspore injection of a single spermatozoon (ICSI) is for the treatment of part of the unexplained infertility and severe male infertility, which is to inject a single spermatozoa into the cytoplasm of the egg through the specially designed micropuncture needle directly. ICSI is a treatment for some cases of unexplained infertility and severe male factor infertility. I. Conventional In Vitro Fertilization – Embryo Transfer (IVF-ET) is suitable for the following conditions: 1. Obstacles to sperm and egg encounter caused by tubal factors. 2. 2. Endometriosis. 3, Unexplained infertility. 4, Immune infertility. 5, Partial ovulation disorders 6, Male oligospermia and hypospermia. Second, single sperm intracytoplasmic microinjection (ICSI) is suitable for the following cases: 1, severe oligo, weak, abnormal spermatozoa. 2.Difficult to cure obstructive azoospermia. 3, Spermatogenesis dysfunction. 4, Immune sterility. 5, Failure of fertilization by conventional in vitro fertilization-embryo transfer. Sperm without acrosome or abnormal acrosome function. 7, Perform preimplantation embryo genetic diagnosis.