In early-stage breast cancer, sentinel lymph node biopsy is a safe and precise procedure that has gradually replaced axillary lymph node dissection as the standard procedure for early-stage breast cancer treatment. With further research, the application of sentinel lymph node biopsy has become more widespread and the postoperative quality of life has improved significantly, but its operation needs to be further standardized. Breast cancer is one of the common malignant tumors in women in China, and its incidence has been increasing year by year. Traditional surgical treatment includes axillary lymph node dissection, which is highly traumatic and has many complications and seriously affects patients’ survival quality. The anterior lymph node is the 1st station regional lymph node of primary tumor metastasis. Recent evidence from evidence-based medicine shows that sentinel lymph node biopsy can accurately reflect the staging of axillary lymph nodes. Some experiences in breast sentinel lymph node biopsy surgery are summarized below. Breast cancer surgery has undergone Halsted radical surgery, extended radical surgery, modified radical surgery and breast preservation, all of which emphasize the contouring of axillary lymph nodes. However, axillary lymph node dissection has many complications, such as upper limb edema, pain, sensory and functional impairment, and whether it prolongs survival is still controversial. Recent studies have shown that most patients with early-stage breast cancer do not have axillary lymph node metastasis, and a significant number of patients with early-stage breast cancer have excessive axillary lymph node dissection in clinical practice. Therefore, the biopsy of anterior lymph nodes has gradually become the main procedure of breast surgery. At present, the positioning of the sentinel lymph node in the majority of primary hospitals mainly relies on the dye positioning method, and there are two main types of dye positioning: (1) melanin; (2) nanocarbon, and the use of these two dyes should pay attention to the following aspects: 1, for breast-conserving surgery patients, attention should be paid to avoid residual skin surface when injecting nanocarbon, because once the nanocarbon remains on the skin surface, it will become a permanent mark; melanin, if injected too shallow, will easily cause non-epidermal necrosis. This should also be noted so that subcutaneous injection should be insisted. Therefore, it is more appropriate to use Melan for breast preservation surgery. According to the latest literature research and clinical practice, it is proved that the biopsy of sentinel lymph nodes should be carried out within 3~5 minutes after injection, so that the lymph nodes will not be overly marked and the detection rate of sentinel lymph nodes will be improved. Regarding the injection site, most scholars and studies believe that the dye should be injected around the patient’s tumor or inside the tumor parenchyma. My working experience is that a four-point subcutaneous injection in the areola is sufficient for accurate labeling of the sentinel lymph nodes. 4. Regarding the localization of the sentinel lymph nodes, for the localization of the sentinel lymph nodes, except for the single mastectomy which does not require another incision, breast-conserving surgery often requires another incision at the lower edge of the axilla, so there is more fatty connective tissue here, especially in obese patients, it is not easy to find the sentinel lymph nodes accurately. The top of the axilla, the outer edge of the pectoralis major, the outer edge of the breast, and the upper edge of the latissimus dorsi muscle form a parallelogram and are equally divided into nine parts, and the lower four parts form a small quadrilateral, where the sentinel lymph nodes are located. 5. Regarding the number of sentinel lymph nodes, it has been reported in the literature that the number of sentinel lymph node biopsies should not be more than 6 lymph nodes, otherwise it indicates that non-sentinel lymph nodes are detected. Currently, sentinel lymph node biopsy is the most frequently used clinical treatment for breast cancer, but the problem of low detection rate and high false detection rate still occurs in clinical practice, so it is necessary to master the correct surgical methods and techniques to improve the accuracy of sentinel lymph node biopsy.