With the development of ultrasound imaging technology, the diagnostic level of breast tumors has been improved, but for some breast masses, even if the diagnosis is still not clear through various imaging methods such as ultrasound, mammography and magnetic resonance, a biopsy is needed to clarify the diagnosis. All breast lesions that can be shown by ultrasound can be localized and sampled under ultrasound guidance. There are two main methods for biopsy of breast lesions, namely fine needle aspiration cytology (FNA) and coarse needle aspiration histopathology. Indications: 1. Identification of benign and malignant masses – all ultrasonically visible intramammary nodules and atypical cysts that are difficult to characterize. 2.Patients with high clinical suspicion of breast cancer. 3.Patients with breast cancer to be treated with breast-conserving surgery or neoadjuvant chemotherapy. 4.The clinical diagnosis of advanced breast cancer is basically confirmed, but the patient is intolerant to surgery and must provide pathological diagnosis for neoadjuvant chemotherapy or endocrine therapy. Contraindications: 1. Hemorrhagic and coagulation disorders. 2.Severe cardiopulmonary disease, severe cachexia. 3. Lesions that cannot be clearly shown by ultrasound or have no safe puncture route. Pre-operative preparation: 1. Pre-operative ultrasound examination, routine ultrasound visible mass or suspicious lesion area, in line with the indications, and safe puncture pathway. 2, The patient (within one week) has undergone routine blood, coagulation function and serological examination with results in the normal range. 3.Aspirin, warfarin and other anticoagulant drugs need to be stopped after one week. 4.The physician needs to talk with the patient before the operation to inform the purpose of the examination and the possible risks, and needs to sign the relevant “Informed Consent Form”. 5. The physician reviews the imaging data before surgery, and ultrasounds again before surgery, and marks the puncture site well. In addition to biopsy, there is now an ultrasound-guided breast mass localization needle puncture, a preoperative operation performed under ultrasound guidance, which is usually localized to some breast lesions that are out of the clinician’s reach, usually relatively small or deep, thus making breast surgery safer and faster. The ultrasonographer first places the guidewire into the breast lesion with a breast localization needle to locate the site and extent of the lesion before the breast surgery; then the surgeon accurately locates the lesion according to the guidewire, thus making a small incision and greatly reducing the amount of normal tissue removed.