Objective To analyze the feasibility, safety, and resection methods of thoracoscopic surgery for intralobar pulmonary septal disease. Methods Retrospective analysis of 17 patients with intralobar pulmonary segregation treated by thoracoscopic surgery from December 2006 to September 2011 in our hospital, including 7 males and 10 females with an average age of 40.3 (14-61) years. The preoperative diagnosis was confirmed by enhanced CT in 9 cases and undiagnosed in 8 cases. The incision was chosen as a three-hole one, and after finding the anomalous artery within the inferior pulmonary ligament, it was usually cut using a linear cutting suture, followed by either pulmonary wedge resection or lobectomy, depending on the situation. Results Seventeen patients underwent successful thoracoscopic surgery with no intermediate openings and no serious postoperative complications. 5 patients chose to undergo pulmonary wedge resection, 4 were successfully completed, 1 was converted to lobectomy; 12 underwent direct lobectomy. The mean operative time was 128(80-170) min, the mean bleeding volume was 80(5-200) ml, the mean postoperative chest tube drainage time was 4.0(2-6) d, and the mean postoperative hospital stay was 7.6(4-11) d. Intraoperative and postoperative pathology confirmed intralobular pulmonary isolation in all patients. Conclusion Thoracoscopic surgery for intralobar pulmonary isolation is safe and feasible, with preference given to wedge resection or segmental resection of the lung when circumstances permit.