Myoglobin is a small molecule pigment protein. Myoglobin is mainly distributed in cardiac and skeletal muscle tissue and has the function of transporting and storing oxygen. However, low myoglobin is generally not considered to be of particular clinical significance and cannot indicate anything. Compared to low myoglobin, high myoglobin is more clinically significant. The human heart muscle and skeletal muscle contain a large amount of myoglobin, while serum and urine contain less, and the test results may show that it is lower. Low myoglobin only represents a decrease in oxygen-binding protein in the myocardium due to myocardial metabolism and is generally not clinically relevant. Usually, myoglobin is used as a reliable indicator of damage to the heart muscle or skeletal muscle, and tests that indicate an increase in myoglobin are mostly seen in patients with early acute myocardial infarction, severe congestive heart failure, and patients with polymyositis, acute muscle injury, myotonic dystrophy, and myasthenia gravis. In addition, patients with renal insufficiency, burns, alcoholism, and prolonged shock will also have elevated myoglobin levels, which need to be taken seriously.