”Routine tests required before IVF treatment. Female: 1. Serum FSH, LH, E2, T, PRL on day 2-3 of menstruation. 2. Ultrasound examination of ovarian follicles and endometrial condition from day 9-10 of menstruation. 3.Routine preoperative examination for female partner includes immune 8, biochemistry 2, TORCH, RPR, blood type, antibody 4, blood routine, urine routine, TCT, mycoplasma, chlamydia examination. Male partner: 1, semen analysis, including semen routine, sperm penetration test and sperm morphology analysis, etc. 2. Chromosome examination if necessary for male infertility. (Azoospermia, severe oligozoospermia) 3. Pre-operative examination of the male partner includes immune 8 items, biochemical 2, RPR, blood type, mycoplasma, chlamydia examination.