Failure to detect bacteria in stool culture usually indicates that the patient does not have a bacterial infection, but it does not discharge other microorganisms such as viruses, parasites and other infections. Stool culture is mainly to culture microorganisms in stool, such as bacteria, fungi, parasites, etc. It can be used to determine the type of microorganisms infecting the patient, and combined with drug sensitivity test, it can help the clinic to determine what kind of antimicrobials should be applied. Usually, when a patient has an intestinal infection such as abdominal pain and diarrhea, nausea and vomiting, fever, etc., the stool culture can be used to determine the specific microorganisms of the infection. When no bacteria are detected in the stool culture, it usually indicates that there is no bacterial infection in the patient’s intestinal tract, but it does not exclude infections by pathogenic microorganisms such as viruses, fungi, and parasites. False negatives should also be ruled out. If the patient applies antibiotic drugs before the test, or if there are substances such as antiseptic solutions in the containers used, or if the test is sent for too long a period of time, this may lead to the death of bacteria and false negatives in the test. If patients have gastrointestinal infections, they should seek medical attention and have stool cultures under the guidance of a doctor in order to carry out targeted treatment to avoid delays.