What are closed antibodies? During pregnancy, closed antibody is an antibody produced by maternal exposure to antigens of paternal origin, which can bind to placental cell surface antigens, thus blocking maternal cytotoxic T cells from launching an immune attack on the embryo and playing a role in protecting the fetus and maintaining pregnancy. The relationship between closed antibody and habitual miscarriage About 80%~90% of women with habitual miscarriage cannot detect this specific closed antibody, and there are uninhibited cytotoxic cells in their bodies. These cells can act directly on the placenta or indirectly damage the fetus or placenta by releasing inflammatory mediators, thus leading to miscarriage. What is Lymphocyte Active Immunotherapy? Lymphocyte Active Immunotherapy involves the injection of the husband’s or a third party’s lymphocytes into the body of a patient with habitual miscarriage to induce an alloimmune response and the acquisition of closed antibodies and microlymphocytotoxic antibodies. In this way, the mother’s immune system does not produce an immune attack against the fetus, improving the success rate of another pregnancy. What are the advantages of activated lymphocyte active immunotherapy over general lymphocyte active immunotherapy?1. A small amount of blood drawn at a time is enough for a full course of treatment. Draw 40ml of blood from husband or third party to separate lymphocytes, activate and proliferate them in vitro, use the proliferated lymphocytes to perform immunization injection, and freeze the remaining lymphocytes for spare use. It can be maintained for about 1 year with more than 10 injections.2. Better efficacy. The activated lymphocytes are more pure and active, and it is easier to stimulate the closed antibody in the patient’s body.3. Ensure healthy supply. When the husband suffers from hepatitis B and other infectious diseases and is not suitable for blood supply, activated lymphocytes can solve the problem of not being able to find a third party to supply blood. What is active immunotherapy with activated lymphocytes? Activated lymphocyte refers to extracting the blood of the husband or the third party, separating the lymphocytes and stimulating them with cytokines in vitro to make them activate and proliferate. The purer and more active lymphocytes are obtained, which are more likely to stimulate the production of closed antibodies when injected into the patient’s body. Applicable people of activated lymphocyte active immunotherapy: 1. Those who cannot turn positive by lymphocyte active immunotherapy, 2. Those whose blood donors are not convenient to come for fresh blood every time, 3. Those whose husbands are suffering from hepatitis B, hepatitis C, AIDS, syphilis, tumors and other diseases, which are not suitable for blood donors. Precautions before treatment: 1. Those who have a history of habitual abortion and are negative for closed antibody (APLA) need to undergo active immunotherapy with lymphocytes. 2. The blood donor should complete the relevant examinations for hepatitis B, hepatitis C, AIDS and syphilis before the patient’s treatment. 3. The blood donor should preferably be the patient’s husband, and if the husband is not suitable to be the blood donor, then he/she can choose the blood donor with his/her husband, then those who are not blood related with the patient, and finally those who have no blood relation with the patient. 4. If the husband is not suitable for the blood donor, the next choice is the person who is related to the husband, the next choice is the person who is not related to the patient, and the last choice is the person who is related to the patient.