Brucellosis, also known as brucellosis, has a certain chance to be detected by agglutination test and enzyme-linked immunosorbent assay during the incubation period, but there is a possibility of false negative. 1. Brucella agglutination test: can be used to diagnose brucellosis, test serum antibodies to Brucella and latex reagent will produce antigen-antibody reaction, causing a visible agglutination reaction. 2. Enzyme-linked immunosorbent assay (ELISA): a widely used technique in enzyme immunoassay technology, in which known antigens or antibodies are adsorbed on the surface of solid-phase carriers, prompting the enzyme-labeled antigen-antibody reaction to proceed on the surface of the solid phase, and then the free components in the liquid phase can be washed with the help of washing method. In addition, brucellosis is also harmful, so in order to avoid affecting health, once diagnosed, patients need to be actively treated under the guidance of specialists.