In vitro fertilization is an important part of assisted reproductive medicine technology, from which in vitro sperm injection and genetic diagnosis before embryo transfer are derived. It is mainly applicable to diseases or factors such as oligozoospermia, severe deformed spermatozoa and azoospermia in the male partner, and in the female partner, mainly bilateral tubal blockage and history of infertility for many years, and older age of the female partner. The basic procedure is to first carry out the female partner’s descending mediation, and then according to the comprehensive analysis of reproductive hormones, age, and physical fitness, to formulate an ovulation promotion program, which there are many kinds of ovulation promotion programs. The size of the developing follicles is then monitored under blood and ultrasound, and if most of the bilateral follicles are larger than 17mm in diameter, an overnight injection is required and the eggs are retrieved 36 hours later. At the same time as the eggs are retrieved, the male partner will have to retrieve the sperm, which will be optimized by the in vitro fertilization laboratory personnel, cultivate the sperm and eggs, mix them in a certain ratio, fertilize them to form an embryo, and after raising them, determine the transfer to the woman’s body in order to help her conceive.