Often transaminases may be elevated with poor rest. The liver is the largest energy supply organ in the body and needs to produce adenosine triphosphate for the body to carry out its activities. If the patient is fatigued for a long time, especially if he/she is not well rested, it may increase the burden of energy supply to the liver, which may lead to liver injury and elevated transaminases. Therefore, patients need to pay attention to rest before the next day’s liver function test, so as not to cause transaminase elevation. Clinically, aminotransferases mainly refer to glutamic oxaloacetic aminotransferase and glutamic propyltransferase. If patients have problems such as staying up all night, fatigue, alcohol consumption and anxiety, it will lead to a slight elevation of aminotransferase levels. Patients with elevated aminotransferase levels should actively seek medical attention and receive standardized treatment under the guidance of a medical professional who can identify the cause of the problem.