Penicillin overuse does not cause deafness. Common ototoxic drugs include aminoglycosides, vancomycin, chloramphenicol, etc. Overuse of the above drugs can cause deafness. Penicillin belongs to β-lactam antibiotics, its antibacterial effect is mainly to destroy the bacterial cell wall, and when the bacterial cell is in the reproduction phase of bacterial sterilization. There are many adverse reactions when this type of antibiotic is applied clinically, including drug fever, rash, angioneurotic edema, and anaphylaxis. Skin tests are required before penicillin application, or drug allergy can be avoided. Antibiotics with ototoxicity mainly refer to aminoglycosides, and common drugs include gentamicin, etilmicin, streptomycin, etc. Vancomycin and chloramphenicol also have some ototoxicity. These drugs often cause deafness after use, while penicillin’s drug description has no information about causing deafness expressed. Overuse of penicillin cannot cause deafness, but it should not be used excessively, and should be used in accordance with the doctor’s instructions, and should not be applied on its own.