Chronic cholecystitis immunohistochemistry may refer to a differential diagnostic process in which patients with chronic cholecystitis are genetically tested for pathological tissue by immunohistochemistry. Immunohistochemistry examination generally refers to a test for pathologic diagnosis by specific staining of tissue sections using the principle of specific binding of immunological antigens and antibodies. It is helpful in the differential diagnosis of malignant tumors, the differential diagnosis of poorly differentiated or undifferentiated tumors, and further pathological typing of certain types of tumors. Chronic cholecystitis is a manifestation of abnormal gallbladder function caused by recurrent episodes of acute or subacute cholecystitis, or long-standing gallbladder stones. Such patients can further undergo immunohistochemical examination, which helps to correctly diagnose whether chronic cholecystitis is malignant or not, and improve the diagnosis rate. It is worth noting that immunohistochemistry has its limitations, and its sensitivity and specificity at the protein level are insufficient; therefore, it should be used in combination with other histochemical and molecular biological techniques.