The anti-o test should be performed by first adding streptavidin O to the patient’s serum, then adding red blood cells from rabbits or sheep, and finally observing whether the red blood cells are lysed.
The patient’s blood specimen is first drawn and the serum is obtained by centrifugation. After that, the patient’s serum was doubly diluted, and a quantitative amount of streptavidin o was added to the doubly diluted patient’s serum, and then rabbit or sheep red blood cells were added. Finally, after a period of standing, the red blood cells were observed for lysis. If the patient’s serum contains antibodies against streptococcal hemolysin o, the red blood cells are not lysed, and if the patient’s serum does not contain anti-o antibodies the red blood cells are lysed.
In addition to streptococcal infection, there are other reasons that may lead to a positive anti-o test, such as hypercholesterolemia, etc., which must be analyzed in combination with clinical symptoms and relevant examinations to make a correct diagnosis.