Haemophilus culture is to check for Haemophilus infection. Haemophilus influenzae is a common clinical pathogen that can cause meningitis, bronchitis, pharyngitis, septicemia of pneumonia, septic arthritis and pericarditis. Haemophilus is the specimen of the lesion site (such as sputum, cerebrospinal fluid) is inoculated into the culture medium where Haemophilus can survive, such as blood plate, MacConkey plate, chocolate plate, etc., and put into the 6% CO2 incubator to culture for 24 h. Observe whether there are colonies on the culture medium and the characteristics of the bacteria, etc. to judge whether there is Haemophilus infection or not. The growth conditions of Haemophilus are relatively harsh, and the culture requires factor V and X. Haemophilus influenzae is a non-powered, non-budding Gram-negative fine bacillus, spherical rod-shaped, filamentous or polymorphic; it is a caustic bacterium with harsh nutritional requirements, and does not grow on ordinary culture medium. Haemophilus culture can be used to diagnose the disease and guide the next step of medication, if found Haemophilus infection is recommended to consult a doctor in time, under the guidance of the doctor for further diagnosis and treatment.