Lymphocentesis showing no definite malignant tissue may be a benign lymph node enlargement, or it may be that no diseased lymph node tissue has been taken. Lymph nodes are not palpable and enlarged in normal people. When unexplained lymph node enlargement occurs in the human body, lymph node aspiration biopsy is feasible to clarify its pathological nature and thus guide clinical treatment. If lymphocyte puncture shows no clear malignant tissue, there are two possibilities: the lymph node enlargement is caused by benign sclerosing etiology, such as tuberculosis and lymphadenitis. Due to the limited amount of lymph node tissue taken by the lymph node aspiration biopsy needle, false negatives may also occur, where no diseased lymph node tissue is taken. At this time, lymph node biopsy pathology examination is feasible, if the lymph nodes are enlarged due to lymphoma, malignant solid tumor lymph node metastasis and other malignant etiology, malignant tissue cells can be found. If no malignant tissue cells are found in lymph node biopsy, then it is clear that the lymph node enlargement is benign. Therefore, it is recommended that patients whose lymphocyte puncture reveals no clear malignant tissue should undergo further lymph node biopsy to make a clear diagnosis and then receive targeted treatment.