Androgen levels are tested by taking blood on an empty stomach. Patients are usually required to come to the hospital between 7-9 am, after having fasted from food and water for about 8 hours, and then have their blood drawn for the test, which consists of the six hormone levels. However, this test can be affected by some factors in life, which can easily cause errors. It is usually recommended that patients should not have violent mood swings, such as anger or anxiety, tiredness, insomnia, or drinking alcohol 1-2 days before the test, and should not do this test after exercise, in order to ensure the accuracy of the test results. The main purpose of testing androgen levels in clinical practice is to determine the condition of the testicles and to infer the cause of impotence, infertility and the development of other endocrine diseases.