The procedure for ultrasound-guided deep vein puncture includes using an ultrasound probe to determine the location and alignment of the deep vein and ultrasound-guided deep vein puncture. Adjust the body position and use bedside ultrasound to find the location of the deep vein to be punctured and determine the puncture point before puncture. Then the operation of wearing gloves, disposable surgical gowns, sterilized spreading towels, and checking the puncture kit is carried out, assisted by an assistant, who wraps the ultrasound probe with sterile gloves under the principle of ensuring asepsis to make sure that the puncture point will not be contaminated by the probe. Ultrasound to find the puncture vessel, the midpoint is placed on the blood vessel, then the left hand fixes the ultrasound probe, the right hand holds the puncture needle slowly into the needle, and look for the tip of the needle on the ultrasound image, when you see the tip of the needle into the target blood vessel and draw out dark red blood, the puncture is successful. The operation is successful when the assistant assists in evacuating the ultrasound probe, placing the guidewire, expanding the skin, placing the tube, and fixing it.