Neonatal neurological infection can be determined based on the nature of cerebrospinal fluid from lumbar puncture, and also based on the general symptoms of neurological infection, such as elevated temperature, irritability, meningeal irritation, and other clinical manifestations for a comprehensive analysis. Neonatal neurological infections may be bacterial or viral infections, and acute bacterial meningitis can be caused when various purulent bacterial infections are present, with acute fever, convulsions, increased intracranial pressure, and meningeal irritation signs as the main clinical symptoms. However, the performance of newborns is mostly atypical, the body temperature may be high or low, the increase in intracranial pressure may not be obvious, there may only be spitting up and other phenomena, in addition to the appearance of facial limbs, mild convulsions, episodes of blinking, breathing irregularly, breath-holding and other manifestations, if the appearance of such symptoms, the newborn should be suspected of neurological infections. When the virus infects the nervous system, there may be irritability, irritability, neck straightening, lethargy and other manifestations, and the infection is usually preceded by upper respiratory tract infections and other conditions. At the same time, lumbar puncture cerebrospinal fluid can be used for examination, can be based on the cerebrospinal fluid pressure, appearance, protein content, etc. Cerebrospinal fluid examination can generally clarify the neurological infection or not and the type of infectious agent. If a newborn baby has symptoms of neurological infection, it is necessary to consult a doctor in time and follow the doctor’s instructions for treatment, so as to avoid delaying the treatment time.