Negative antinuclear antibodies indicate that the patient can basically rule out the possibility of SLE and is less likely to suffer from other autoimmune diseases at the same time. Antinuclear antibody is an autoantibody that uses the nucleus of human cells as antigen, and the spectrum of antinuclear antibody includes anti-dsDNA antibody, anti-SSA antibody, anti-Scl-70 antibody, anti-nodal antibody, etc. The clinical significance is greater when the titer of antinuclear antibody is ≥1:80. Antinuclear antibody is an important marker for autoimmune diseases, and its positivity indicates autoimmune diseases such as systemic lupus erythematosus (SLE), mixed connective tissue disease (MCD), desiccation syndrome (DS), systemic sclerosis (SSS), and polymyositis (PMI), and its negativity suggests that the chances of suffering from the above diseases are small. The antinuclear antibody positivity rate for SLE is about 90% or more, and a negative test basically excludes the possibility of SLE. Laboratory reports need to be interpreted by a professional physician, and it is recommended that patients with negative antinuclear antibodies go to the Department of Rheumatology and Immunology of a regular hospital for detailed consultation.