The male semen is mainly a biological environment consisting of a large amount of water, part of sperm, proteins and enzymes, so once the sperm is out of this watery environment, it should enter the birth canal in time. In this case of separation from the body, once the spermatozoa have lost their water, it will not take more than two hours to completely lose their biological function. Therefore, if in vitro sperm preservation is to be performed, the sperm should be given nutritional support and a water environment in a timely manner. Sperm will not have any biological function at all after drying. Generally, sperm will survive for 72 hours after discharge into the female birth canal, and will not have any conception function after this time. Therefore, the male should maintain a good water environment when producing sperm in order to maintain the biological function of sperm and the normal transmission of chromosomes.