The H. pylori self-test paper method has a high rate of misdetection and its accuracy is not as good as the urea breath test in hospitals. Since H. pylori can secrete urease, the presence or absence and strength of urease can reflect the presence or absence and number of H. pylori. Based on this principle, a rapid urease reaction test paper, i.e. H. pylori self-test paper, was designed. If the gastric mucosa tissue contains urease, which can decompose urea to produce alkali, and the reaction membrane phenol red indicator shows red, then H. pylori can be detected. However, the operation process of self-testing should be strictly in accordance with the instructions for proper operation, improper operation may result in a high rate of false-positive or false-negative tests. In conclusion, the results of H. pylori self-testing paper can only be used as a general screening for reference, and cannot be used as a basis for diagnosis, because the accuracy of the results of self-testing with test strips is also affected by the quality of the test strips, the representativeness of the samples taken, and the standardization of the operation, and many other factors. Just like the early pregnancy test, further standardized examination in the hospital is required for final confirmation, please follow the doctor’s instructions. In addition, the H. pylori self-test paper should be kept dry to prevent the paper from turning red when wet, which may affect the accuracy of the result.