How much it costs to test for B27 has a lot to do with local prices and how the test is performed. In some areas, a flow cytometry test usually costs about$130.00. The full name of B27 is HLA-B27, and positivity for this gene is primarily associated with spondyloarthritis. Often, a young male with inflammatory lower back pain or peripheral joint pain, iritis, heel pain, etc., combined with B27 positivity suggests the presence of spondyloarthritis. However, B27 positivity does not always lead to the development of spondyloarthritis; some patients can carry it for a long period of time and it does not lead to the development of the disease. In addition, B27 negativity does not rule out spondyloarthritis; it is only one indicator of spondyloarthritis and requires a combination of symptoms, physical examination, and medical history to diagnose spondyloarthritis.