The normal value of CD34 possessed nucleated cells is 1% to 4% in human bone marrow and 0.1% to 0.3% in human peripheral blood. CD34-positive cells are mainly some primitive cells, and if the test index exceeds the range of normal values, it often suggests the possible existence of leukemia and other diseases. Clinically, CD34 positive cells can be used to represent hematopoietic stem cells, and clinical testing of CD34 positive cells is mainly for hematopoietic stem cell transplantation or bone marrow transplantation to provide an important basis. Generally, the number of CD34-positive cells can be used to select the appropriate time to collect hematopoietic stem cells, and can be used as a basis for the use of growth factors.