Preface: Semen quality can be influenced by many factors and is highly volatile. Therefore, if a problem is found in the first test, the regular testing facility will often ask the patient to do another test or even several tests, and several times the results match before making a judgment. The average number of sperm drops significantly when you are emotionally stressed There was a patient who was found to be azoospermic during the first test, but the number of sperm in his semen was very good the second time he had the test. After careful questioning by the examiner, it turned out that the patient was very nervous during the first sperm retrieval. If a couple, married for many years without having children, goes to the hospital to see a doctor. The doctor will have the nurse give the man a small cup and take him to a special bathroom and let him in. Inside there are chairs and pornographic magazines. The nurse tells him to get the semen out and give it to her. She closes the door and waits just outside. Many men were shy and embarrassed, nervous at the thought of the nurse lady outside this door for a long time. But others did not take it seriously and finished the task with ease. The doctors, without letting the patient know, compared the time it took one hundred and forty-two people to complete the task and the quality of their semen, and found that the average number of sperm per milliliter was 78 million for those who handed over their samples in five minutes. And those who were emotionally stressed and took more than fifteen minutes to complete the task had only 28 million sperm per milliliter of semen. According to the standards of the World Health Organization, to be able to conceive a woman, the concentration of sperm must be at least 20 million per milliliter. Why do emotions affect the quality of human semen? Doctors explain that normal sex impulses are transmitted from the hippocampus of the brain to the pituitary gland and down to the testes. The testes produce androgens that not only regulate sperm production, but also affect the muscular contraction of the vas deferens. When a person is in a state of stress and anxiety, this channel of information transmission is blocked, which not only affects orgasm, but also prevents many sperm from being released into the body. What is the best method of sperm retrieval? Currently, sperm retrieval relies on the patient’s own massage and stimulation to achieve ejaculation, and hospitals have special sperm retrieval rooms. However, some patients are easily influenced by their environment, the more educated they are, and some of these people offer to take their sperm home, but end up taking interrupted sex instead. Semen samples taken in this way are often unreliable, as the semen from the anterior segment is likely to have flowed in the female vagina, making it impossible to obtain an ideal sample for testing. Patients are advised to try to obtain semen in the hospital. When taking semen, soft soap or paraffin oil can be used to do penile massage and collect the specimen in a sterile test tube; condoms (rinsed clean and free of spermicidal drugs) or interrupted intercourse methods can also be used to collect semen, but the amount collected in this way is often small. There are often two ways to collect semen, one way is for the patient to come to the hospital, ask the examiner for a clean (mostly sterilized) bottle or cup to keep the semen, and later go to the sperm room and masturbate to remove the semen. If the patient is unable to masturbate, the second method is to have intercourse in the morning, expel semen in vitro, and retain semen in a glass tube or clean glass bottle. Try not to use condoms to retain semen, because the chemical composition of condoms has an effect on sperm, so as not to affect the accuracy of the test results. After taking the semen, it is advisable to hand it over to the examiner within half an hour After taking the semen, it should be sent to the examiner immediately, especially if the semen is taken at home, it is best not to take more than half an hour to send it to the examiner because the examiner has to smear the semen specimen immediately after receiving it to observe the presence of sperm and sperm motility, the percentage of active sperm and morphology under the microscope, because the sperm has been out of the body for too long, especially In winter, sperm motility and morphology will be affected. Therefore, in winter, after the sperm has left the body, on the way to the examination, attention should be paid to insulation, preferably in a test tube, placed in the pocket of the underwear, in order to keep warm and avoid sperm death. It has been found that many semen specimens, not handed directly to the examiner, when the examiner finds the specimen smear examination, the semen has been liquefied and the sperm all dead. The examiner does not know how long the semen has been out of the body and does not know if this death is due to natural death from a long period of time out of the body or if it was already dead when it was out of the body. The examiner can only make a report based on the observed objective results, which will inevitably have an impact on the diagnosis and treatment of the patient. Therefore, special emphasis should be placed on the timely delivery of semen, and it should be handed over to the inspector, who should immediately make an examination to ensure the reliability of the test results. In cases such as the above, the examiner does not know whether the sperm is naturally dead or pathologically dead, and the clinician may be wrong in diagnosing and treating the patient based on this report, which may actually be caused by the patient himself. Therefore, it is important that patients follow the requirements for semen retention and delivery.