The choice of chemotherapy regimen for two-positive and one-negative breast cancer needs to be decided according to the patient’s pathological type and immunohistochemistry results, and commonly used chemotherapy regimens include anthracycline-containing chemotherapy regimen, paclitaxel chemotherapy regimen and so on. 1. Anthracycline-containing chemotherapy regimen: anthracycline-containing drugs refer to a class of drugs containing anthracycline, which belongs to the class of antitumor antibiotics, and the main ones used in chemotherapy for breast cancer are adriamycin, epirubicin and pirenzolubicin. Commonly used combination chemotherapy programs are CAF program, such as cyclophosphamide+doxorubicin+fluorouracil; CEF program, such as cyclophosphamide+epirubicin+fluorouracil. 2. Paclitaxel chemotherapy regimen: Paclitaxel is a new type of anti-microtubule chemotherapeutic drug, which can cause microtubules to aggregate into clusters and bundles, and inhibit the normal reorganization of microtubule network. Paclitaxel+carboplatin is one of the commonly used chemotherapy regimens in the clinic, and attention should be paid to recheck the blood routine after chemotherapy to observe the bone marrow suppression. The chemotherapy regimen for two-positive and one-negative breast cancer should be carried out in accordance with the doctor’s prescription, and the best chemotherapy regimen should be chosen to extend the patient’s life and reduce the patient’s pain to the longest extent.